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  • Nicosia, Cyprus
  • Mon – Fri: 8:30 am – 5:00 pm,
    Sat – Sun: Closed
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ICSI Treatment

(Intracytoplasmic Sperm Injection)

ICSI (Intracytoplasmic Sperm Injection) is one of the most effective techniques available in assisted reproduction today. It is primarily designed to overcome male factor infertility, but it’s also widely used in cases of low egg quality, fertilization failure, or frozen egg use.

At Pearl Fertility, ICSI is included as a standard part of all IVF cycles — at no additional cost — to maximize your chances of successful fertilization.

What is ICSI?

Fertilization naturally occurs when a healthy sperm penetrates the outer layer of the egg and reaches its cytoplasm. However, sometimes sperm may not be able to do this due to:

  • Low motility
  • Abnormal shape
  • Genetic or structural issues

ICSI bypasses this barrier by directly injecting a single healthy sperm into the center of the egg using a microscopic needle — a precise procedure done by skilled embryologists in the IVF laboratory.

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Advanced Techniques: ICSI + IMSI

In addition to standard ICSI, we also apply IMSI (Intracytoplasmic Morphologically Selected Sperm Injection). This advanced technology allows the embryologist to select the healthiest and most structurally normal sperm under high magnification, improving fertilization outcomes — especially for men with abnormal sperm morphology.

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Who Can Benefit from ICSI?

ICSI is recommended in cases of:

  • Male infertility (low count, motility, or morphology)
  • Fertilization failure in previous IVF cycles
  • Use of frozen or in vitro matured eggs
  • Low egg quality

Unexplained infertility

The ICSI Treatment Process – Step by Step

1.

Evaluation

  • Review of both partners’ medical history and any previous IVF attempts
  • Recommendation to bring past test results or treatment reports
  • Initial blood tests and screening for both partners

2.

Fertility Testing & Imaging

  • Hysterosalpingography (HSG): An X-ray procedure to check for blocked fallopian tubes and evaluate uterine structure
  • Ultrasound scan: To assess ovarian reserve and uterine environment
  • Hormone blood tests (AMH, FSH, Estradiol, TSH, Prolactin)
  • Semen analysis and infectious disease screening for male partner

3.

Personalized Medical Protocol

  • Hormonal stimulation begins on day 2 or 3 of the menstrual cycle
  • Injectable medications stimulate follicle growth over ~10 days
  • Ultrasound monitoring every few days to track follicular development
  • Protocols are customized for each patient based on response, age, and medical background

4.

Egg Retrieval & Fertilization

  • Once follicles are mature, a trigger injection is given 36 hours before egg collection
  • Blood tests (estradiol and progesterone) determine if a fresh or frozen embryo transfer is more suitable
  • On retrieval day, the patient must fast for 6 hours (light sedation is used)
  • Sperm sample is collected the same day (3–5 days abstinence recommended)
  • Embryologists perform ICSI/IMSI in the lab to fertilize the eggs
  • Patients receive updates on fertilization and embryo development

Embryo Transfer

  • Performed 3–5 days after retrieval, depending on embryo quality
  • The patient arrives with a full bladderto assist with ultrasound guidance
  • The procedure is painless and done while the patient is awake
  • After transfer, you’ll receive:
  • A detailed medication plan
  • A post-transfer care guide
  • Clear instructions on what to do and avoid

A pregnancy test (HCG blood test) is done 12 days after the transfer.

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Duration of Stay in Cyprus

Option 1: 20-Day Full Stay

  • Begin treatment in Cyprus from day 1 or 2 of the menstrual cycle
  • All scans and medications are administered at the clinic
  • Best for convenience and full clinical supervision

Option 2: 10-Day Partial Stay

  • Begin stimulation and early scans in your home country
  • Travel to Cyprus around day 10, before the trigger injection
  • Ideal for those who prefer to minimize time abroad
  • We assist with remote monitoring, prescriptions, and pharmacy coordination

Required Medical Tests (Before Starting ICSI)

  • For Female Partner:
    • Hormonal panel: AMH, FSH, LH, Estradiol, TSH, Prolactin
    • Infectious diseases: HIV, Hepatitis B & C, Syphilis
    • Vaginal swab & Pap smear
    • Ultrasound scan
    • HSG (especially after failed cycles)
For Male Partner:
  • Semen analysis (2–5 days abstinence)
  • Infectious disease panel

Need Help Getting Started?

If you’re considering ICSI in Cyprus or need help understanding your fertility treatment options, we’re here to walk with you every step of the way. From clinic coordination to emotional support — you don’t have to do this alone.

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